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Article in English | LILACS | ID: lil-245926

ABSTRACT

A new method for the determination of branched-chain alpha-ketoacid concentration using lactate dehydrogenase (E C 1.1.1.27) isozyme C4 (LDH) C4) from mouse testes is proposed. The assay is performed on urine and plasma without previous treatment. Alpha-ketoglutarate and pyruvate are determined on the same sample using glutamate dehydrogenase (GDH,EC 1.4.1.2) and lactate dehydrogenase isozyme A4 (LDH5) respectively and subtracted from the total alpha-ketoacid concentration obtained with LDH C4. This value corresponds to the branched chain alpha-ketoacid. Results were linear within the concentration range 8 to 170 mumoles/L. Detection limit was 8 mumoles/L. Analytical recovery was higher than 91 per cent. For microplate assays, recoveries were higher than 84 per cent and the detection limit was 20 mumoles/L. Determinations performed with GDH, LDH A4 and LDH C4 allow differentiation of E3 deficiency from other clinical phenotypes of maple syrup urine disease. The method is simple and fast, and adaptation to microplates would allow screening of newborns.


Subject(s)
Adult , Humans , Female , Child , Child, Preschool , Adolescent , Animals , Rats , Clinical Enzyme Tests , L-Lactate Dehydrogenase/blood , L-Lactate Dehydrogenase/urine , Maple Syrup Urine Disease/diagnosis , Multienzyme Complexes/blood , Multienzyme Complexes/urine , Chromatography, Gas , Glutamate Dehydrogenase/analysis , Maple Syrup Urine Disease/genetics , Sugar Alcohol Dehydrogenases/analysis , Testis/enzymology
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